Isabelle Junot was born in New York to a French father, Philippe Junot and Danish mother, Nina Larsen. Fluent in English, French, Danish and Spanish she moved around Europe her whole life growing up mainly in Spain. She attended Institute Le Rosey, an international boarding school in Switzerland where she studied Theatre Arts and won the award for best actress as the main character in Ionesco's Bald Soprano. She continued her studies in Drama and Film in the University of Virginia.